KMID : 0380620230550050455
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Korean Journal of Food Science and Technology 2023 Volume.55 No. 5 p.455 ~ p.463
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Enzymatic biotransformation-based production of compound K using different parts of ginseng sprouts, validation of analysis methods, and assessment of antioxidant activity
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Seo Hyun-Ji
Song Chae-Yeon Yu Si-Eun Yang So-Won Hwang Da-Eun Jo Hae-Bin Chun Su-Hyun Suh Hee-Jae
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Abstract
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The leaves, stems, and roots of ginseng sprouts were hydrolyzed using ¥â-glycosidase, and the content of ginsenosides and antioxidant properties were compared. Compound K, a deglycosylated form of Rb1 and Rd, was not detected in the leaves, stems or roots before enzymatic hydrolysis. However, after enzymatic hydrolysis, Compound K was generated when reacted for 24 h and 48 h with four different enzyme concentrations (2% Rapidase C80max, 5% Rapidase C80max, 2% Pyr-flo, and 5% Pyr-flo). The highest amount of compound K was produced when the leaves were treated with 2% Pyr-flo for 48 h, reaching 7.01 mg/g. Moreover, the total polyphenol and flavonoid contents, ferric reducing antioxidant power (FRAP) activity, and 2,2¡Ç-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity were all significantly higher in the group treated with 2% Pyr-flo for 48 h than in the other treatment groups (p<0.05). These results provide valuable foundational data for food processing method development using ginseng sprouts.
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KEYWORD
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ginseng sprouts, enzymatic hydrolysis, ginsenoside, method validation, antioxidant activity
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